Tips and tricks for individual in vitro culture of bovine embryos

Azaridolatabad, Nima
Faculteit Diergeneeskunde
Vakgroep Interne Geneeskunde, Voortplanting en Populatiegeneeskunde
Gezamenlijk doctoraat
Nima AzariDolatabad was born on the 21th of March 1987 in Yasuj, Iran. He completed his high school in 2005 majoring in Empirical Science at the Deh-Khoda High School in Yasuj. Iran. In 2012 he obtained the diploma of Bachelor of Science in Animal Science at the Yasuj University in Yasuj, Iran. He obtained the Master diploma of Animal Physiology at the Isfahan University of Technology in Isfahan, Iran in 2014. During his master he worked at the Department of Reproduction and Development, Reproductive Biomedicine Centre, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. His master’s thesis was his first experience with research on oocytes and embryos in sheep. Therefore he published his first scientific paper entitled” Effects of cilostamide and/or forskolin on the meiotic resumption and development competence of growing ovine oocytes selected by brilliant cresyl blue staining” in Theriogenology in 2016. After learning English, he applied to get a PhD position abroad, to deepen his knowledge on working with assisted reproduction in the bovine model. He was accepted as a Joint PhD student at Ghent University and Antwerp University in 2018. During his PhD, he supervised bachelor and master students, and took part in experiments of other PhD students. He followed several specialist courses and is (co-)author of multiple scientific publications.
Academische graad
Doctor in de diergeneeskundige wetenschappen
Taal proefschrift
Prof. dr. Ann Van Soom, Faculty of Veterinary Medicine, Ghent University - Prof. dr. Jo Leroy, Department of Veterinary Sciences, University of Antwerp - Dr. Osvaldo Bogado Pascottini, Faculty of Veterinary Medicine, Ghent University

Korte beschrijving

Assisted reproductive technologies (ART) are the solution to many fertility problems in humans and animals. The use of animals as models for the study of human ART has been fundamental to its development. The cow and the human share a few important reproductive characteristics, making this species an excellent model for human ART The general objective of the present thesis is to improve the current individual in vitro embryo production system in cattle. Today, the best results are obtained with the group culture system, in which 40-50% of the in vitro fertilized oocytes reach the blastocyst stage. Individual maturation, fertilization and embryo culture has the advantage that the final result of in vitro embryo production (blastocyst rate and quality) can be directly related to the original oocyte (ovarian origin, maturation conditions with or without addition of follicular fluid, cumulus oocyte morphology and cumulus expansion). We studied the effect of different concentrations of follicular fluid during oocyte maturation on cumulus expansion, embryo development and quality in an individual culture system. The follicular fluid provides the ideal microenvironment to support the growth and maturation of the oocyte. The effect of supplementing follicular fluid on the maturation medium in a completely individual culture system has not yet been studied. We hypothesized that supplementing in vitro maturation (IVM) medium with follicular fluid would improve embryo development and quality under individual and group culture conditions. We could indeed show that 5% of follicular fluid supplementation during IVM improved the cumulus expansion and the blastocyst development and quality in an individual culture system.


Dinsdag 28 februari 2023, 16:30
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